Pre-sterilised rye grains with a self-healing injection port for mushroom cultivation
It is recommended not to buy this product until you have a colonised agar plate, spore syringe or liquid culture. This product is intended to be inoculated upon arrival.
GENERAL INFORMATION
When we make our grains, every step of the process has been carefully thought out to ensure the highest rate of success.
Why buy from us?
100% clean, reputable Organic Rye grains
Soaked for 12h in chlorine-free water
Washed 5 times to ensure a thorough cleaning
5% Gypsum added to avoid clumping
Simmered for 10 minutes to soften the grains and make it easy for the mycelium to digest the grains
Cooled down slowly over a period of 5 hours to prevent the autoclave from sucking up contaminated air when equalising the pressure
Pre-sealed bags prior to sterilisation
Autoclave tape is used to ensure the sterility process has been effective
Ensured grains have not been sprayed with fungicide. We obtain it from a reputable supplier
Our grains are intact and free of breakages after sterilisation as exposed grains can increase the chances of contamination
Grains prepared at optimum moisture field capacity of around 50%
Self-healing injection port included with every bag
Bags are assured 100% contaminant free due to their sterilization process of 3 hours at 20 psi in a commercial autoclave. Under certain circumstances, there can still be contamination, which could have occurred while the product was being shipped. In the unlikely event that you see contamination in your bag that may be due to shipping, please send us a photo within 2 weeks of receiving the item for a full refund or replacement.
Your bags are made to order; please allow 2 to 3 days of handling time.
Bag Size: 10.16cmX 7.62cmX 45.72cm
Filter Size: 3.81cmX 3.81cm
Brand of bag- Unicorn (4-T)
How to avoid contamination-
Take the bags out of their packaging as soon as possible, and keep them at room temperature, away from direct cold/heat sources. The bags need to breathe and should not be kept where there is no free airflow. Keeping the bag in a closed environment will result in anaerobic contaminants thriving and making the bag mouldy.
We recommend that the bags are used as soon as possible to minimize the chances of contamination.
Work in front of a flow hood or a still air box using a completely sterile workspace.
Sanitise the injection port, flame the needle until red, let it cool, and then inject the bag with 10cc Liquid culture or a completely sterile spore syringe. Do not let the liquid pool at the bottom of the bag. Rotate the bag to disperse the liquid.
Incubate at 24 degrees Celsius in a dark spot and keep all the grain bags at a distance from each other to avoid excess heat, which leads to contamination.
Once inoculated, agitate when 30-50% colonised with clean hands and do not touch the air filter or make the air filter wet.
If the injection site begins to show contamination, that means either your Liquid culture or Spore syringe had contamination in it.
Additional tips-
Recommended for inoculating with Liquid culture 10cc, in contrast to a spore syringe, which may be full of bacterial contamination (Spore syringes are best when purified with Agar plates first, please adhere to this).
Use the inoculated grains/fully colonised grains within 14 days to avoid loss of vigour and dieback.
Always keep the Grains somewhere dry. If the air filter gets wet, the contaminants can start growing on it.
LIQUID CULTURES NOT INCLUDED
This item does not include mycelium. Check out our other listings to add mycelium Liquid cultures to your order. We also offer millet grain spawn.
It is recommended not to buy this product until you have a colonised agar plate, spore syringe or liquid culture. This product is intended to be inoculated upon arrival.
GENERAL INFORMATION
When we make our grains, every step of the process has been carefully thought out to ensure the highest rate of success.
Why buy from us?
100% clean, reputable Organic Rye grains
Soaked for 12h in chlorine-free water
Washed 5 times to ensure a thorough cleaning
5% Gypsum added to avoid clumping
Simmered for 10 minutes to soften the grains and make it easy for the mycelium to digest the grains
Cooled down slowly over a period of 5 hours to prevent the autoclave from sucking up contaminated air when equalising the pressure
Pre-sealed bags prior to sterilisation
Autoclave tape is used to ensure the sterility process has been effective
Ensured grains have not been sprayed with fungicide. We obtain it from a reputable supplier
Our grains are intact and free of breakages after sterilisation as exposed grains can increase the chances of contamination
Grains prepared at optimum moisture field capacity of around 50%
Self-healing injection port included with every bag
Bags are assured 100% contaminant free due to their sterilization process of 3 hours at 20 psi in a commercial autoclave. Under certain circumstances, there can still be contamination, which could have occurred while the product was being shipped. In the unlikely event that you see contamination in your bag that may be due to shipping, please send us a photo within 2 weeks of receiving the item for a full refund or replacement.
Your bags are made to order; please allow 2 to 3 days of handling time.
Bag Size: 10.16cmX 7.62cmX 45.72cm
Filter Size: 3.81cmX 3.81cm
Brand of bag- Unicorn (4-T)
How to avoid contamination-
Take the bags out of their packaging as soon as possible, and keep them at room temperature, away from direct cold/heat sources. The bags need to breathe and should not be kept where there is no free airflow. Keeping the bag in a closed environment will result in anaerobic contaminants thriving and making the bag mouldy.
We recommend that the bags are used as soon as possible to minimize the chances of contamination.
Work in front of a flow hood or a still air box using a completely sterile workspace.
Sanitise the injection port, flame the needle until red, let it cool, and then inject the bag with 10cc Liquid culture or a completely sterile spore syringe. Do not let the liquid pool at the bottom of the bag. Rotate the bag to disperse the liquid.
Incubate at 24 degrees Celsius in a dark spot and keep all the grain bags at a distance from each other to avoid excess heat, which leads to contamination.
Once inoculated, agitate when 30-50% colonised with clean hands and do not touch the air filter or make the air filter wet.
If the injection site begins to show contamination, that means either your Liquid culture or Spore syringe had contamination in it.
Additional tips-
Recommended for inoculating with Liquid culture 10cc, in contrast to a spore syringe, which may be full of bacterial contamination (Spore syringes are best when purified with Agar plates first, please adhere to this).
Use the inoculated grains/fully colonised grains within 14 days to avoid loss of vigour and dieback.
Always keep the Grains somewhere dry. If the air filter gets wet, the contaminants can start growing on it.
LIQUID CULTURES NOT INCLUDED
This item does not include mycelium. Check out our other listings to add mycelium Liquid cultures to your order. We also offer millet grain spawn.
* All products may not be available at all stores, countries and regions.
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Rootlab
28/12-22 Newington RoadMARRICKVILLE, New South Wales 2204
Phone: +61416399905
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