Expand your own Liquid Culture mix Dextrose and Light Malt Extract (DLME)
This product has Corn Dextrose and Light Malt Extract. This is one of the best recipes to expand your own liquid as opposed to using just Honey, Karo or Glucose Syrup.
Explanation for using Light Malt Extract: When inoculating a grain spawn, it colonises much faster since the mycelium has been predisposed to the sugar from grain (In this case, barley). In the same way, when making Malt Extract Agar Plates (MEA), the colonization is considerably faster as the mycelium is already used to the sugar.
Explanation for using Dextrose: These ingredients provide sugars and an assortment of basic minerals, vitamins, and salts helpful in the growth of the mushroom Mycelium. Dextrose makes a clear liquid culture (not cloudy), which makes it easier to spot contamination.
Your purchase includes:
– 100gm of Dextrose Light Malt Extract Mix
Note: Mycelium or Liquid Culture Jars are not included. Check out our other listings to add Mycelium and Liquid culture jars with Injection port and air filter vent. You can use these to expand your liquid cultures at home.
Water Ratios
For every 10gm DLME, add 500ml of water
100gm can make up to 5 Litres of Liquid cultures
Instructions
The mix needs to be sterilized before use :
Sterilize in an Autoclave at 15psi for 20 minutes or Microwave for 2 minutes 3 times.
Ensure the Marble or Stirrer is inside the jar before sterilizing your Liquid culture medium.
Once sterilization is complete, cool your jar until it reaches room temperature.
Inoculate the Jar with clean Liquid culture and keep it in a dark space for 1-2 weeks for it to colonize completely.
The Jar needs to be stirred using a magnetic stirrer (5 minutes) or using your marble to break up the mycelium in swirl motions 5-10 times.
Be careful not to break your glass mason jars when swirling with Marble.
Some sedimentation might be present at the bottom of the jar after sterilisation. It is normal.
Use
The liquid culture mix can be used in many different cultivation techniques. Once you have prepared your LC mix, you may inject the liquid culture into sterilized grain jars/bags, agar Petri dishes, or even another jar containing LC mix to create more liquid culture. For aggressive strains (like oysters, Reishi) you can even use it to colonize substrates directly.
This product has Corn Dextrose and Light Malt Extract. This is one of the best recipes to expand your own liquid as opposed to using just Honey, Karo or Glucose Syrup.
Explanation for using Light Malt Extract: When inoculating a grain spawn, it colonises much faster since the mycelium has been predisposed to the sugar from grain (In this case, barley). In the same way, when making Malt Extract Agar Plates (MEA), the colonization is considerably faster as the mycelium is already used to the sugar.
Explanation for using Dextrose: These ingredients provide sugars and an assortment of basic minerals, vitamins, and salts helpful in the growth of the mushroom Mycelium. Dextrose makes a clear liquid culture (not cloudy), which makes it easier to spot contamination.
Your purchase includes:
– 100gm of Dextrose Light Malt Extract Mix
Note: Mycelium or Liquid Culture Jars are not included. Check out our other listings to add Mycelium and Liquid culture jars with Injection port and air filter vent. You can use these to expand your liquid cultures at home.
Water Ratios
For every 10gm DLME, add 500ml of water
100gm can make up to 5 Litres of Liquid cultures
Instructions
The mix needs to be sterilized before use :
Sterilize in an Autoclave at 15psi for 20 minutes or Microwave for 2 minutes 3 times.
Ensure the Marble or Stirrer is inside the jar before sterilizing your Liquid culture medium.
Once sterilization is complete, cool your jar until it reaches room temperature.
Inoculate the Jar with clean Liquid culture and keep it in a dark space for 1-2 weeks for it to colonize completely.
The Jar needs to be stirred using a magnetic stirrer (5 minutes) or using your marble to break up the mycelium in swirl motions 5-10 times.
Be careful not to break your glass mason jars when swirling with Marble.
Some sedimentation might be present at the bottom of the jar after sterilisation. It is normal.
Use
The liquid culture mix can be used in many different cultivation techniques. Once you have prepared your LC mix, you may inject the liquid culture into sterilized grain jars/bags, agar Petri dishes, or even another jar containing LC mix to create more liquid culture. For aggressive strains (like oysters, Reishi) you can even use it to colonize substrates directly.
* All products may not be available at all stores, countries and regions.
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